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Topic 428 - Hair Multiplication & Other Advanced Treatments (forum # 34)

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Another post worth looking at

From: Kevlar
Date: 28 Jun 2001
Time: 02:03:57
Remote Name: 140.142.181.32

Comments

Here is another article that explains a RU58841 study but concealed within is the procedure for culturing dermal papilla cells and outer root sheath cells. This might be interesting to those of you who started the DP culture kits. This is also an interesting article on RU58841. http://endo.endojournals.org/cgi/content/full/138/1/356?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=RU-58841&searchid=993715820160_288&stored_search=&FIRSTINDEX=0&journalcode=endo

Coculture of dermal papilla cells and outer root sheath cells Dermal papilla cells were inoculated at a density of 1 x 104 cells/well into type I collagen-coated multiplates (24 wells) (Corning, New York, NY) and cultured with DMEM supplemented with 10% charcoal-treated FBS. After a 24-h incubation, the medium was discarded and outer root sheath cells (1.5 x 104 cells/well) were added with KGM without bovine pituitary extracts. Further, bald frontal dermal papilla cells from postpubertal macaques and outer root sheath cells were cocultured using transwells (Becton Dickinson, Bedford, MA) to determine which type of cell proliferation was stimulated by coculture or inhibited by testosterone. Some outer root sheath cells were cultured on the membrane of transwells coated with type I collagen and those were placed in the wells in which dermal papilla cells had been cultured on the bottom.

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From: party poker
Date: 11 Mar 2006
Time: 14:54:43
Remote Name: 68.87.66.150

Comments

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